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Updated: Oct 9, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Nucleotide sequence of the pirital virus (family Arenaviridae) small genomic segment
R N Charrel1, X de Lamballerie, P De Micco
1Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA. rnc-virophdm@gulliver.fr
Abstract:
Pirital virus is a newly discovered South American member of the family Arenaviridae. We determined that the complete nucleotide sequence of the small genomic segment of Pirital virus is 3393 nt long, and encodes the viral nucleoprotein (N) and glycoprotein precursor (GPC) (561 aa and 509 aa, respectively) in nonoverlapping open reading frames of opposite polarities. The N and GPC genes are separated by an intergenic region that is 80 nt long; the predicted secondary structure of this region includes a single hairpin stabilized by 11 G-C and 8 A-U base pairs. Independent analyses of N and GPC amino acid sequence data confirmed that Pirital virus is related to Pichindé virus and belongs to the lineage A of the New World (Tacaribe complex) arenaviruses. The analysis of genetic distances between Pirital virus and other arenaviruses confirmed that Pirital virus is a distinct species within the family Arenaviridae.
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