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Updated: Jul 9, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

From ligand to response: generating diversity in nuclear receptor coregulator function

N J McKenna1, B W O'Malley

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, 77030, Houston, TX, USA.

The Journal of Steroid Biochemistry and Molecular Biology
|February 13, 2001
PubMed

Insights

Nuclear receptor ligands control biological processes by activating nuclear receptors. Coactivators are key factors in this process, mediating the effects of these ligands and influencing gene expression.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Genetics

Background:

  • Nuclear receptors are crucial intracellular proteins regulating gene expression.
  • Ligands binding to nuclear receptors modulate diverse physiological functions.
  • The precise mechanisms of nuclear receptor-mediated gene regulation are under investigation.

Purpose of the Study:

  • To review recent advancements in understanding nuclear receptor function.
  • To explore the role of coactivators in nuclear receptor signaling.
  • To discuss the pharmacological implications of coactivators in nuclear receptor ligand action.

Main Methods:

  • Literature review of recent studies on nuclear receptors and coactivators.
  • Analysis of mechanistic aspects of receptor-coactivator interactions.
  • Discussion of pharmacological relevance based on current research.

Main Results:

  • Nuclear receptors, upon activation by ligands, recruit diverse coactivator proteins.
  • Coactivators are essential for efficient transcriptional regulation by activated nuclear receptors.
  • These interactions form the basis for the complex pharmacology of nuclear receptor ligands.

Conclusions:

  • Coactivators play a central role in mediating the biological effects of nuclear receptor ligands.
  • Understanding coactivator function is critical for developing targeted therapies.
  • Further research into receptor-coactivator mechanisms will illuminate nuclear receptor pharmacology.

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