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Updated: Jul 19, 2026

In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
SRC transcriptional activation in a subset of human colon cancer cell lines
1Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
Abstract:
Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.
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