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Identification of a novel DRB1 allele, DRB1*1112, by sequence-based DRB typing
M Luo1, J Blanchard, I Maclean
1Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Tissue Antigens
|February 13, 2001
Abstract:
We report here a novel DRB1 allele (DRB1*1112) identified during sequence-based HLA-DRB typing. Polymerase chain reaction with generic DRB primers and group-specific primers and subsequent sequencing yielded identical results. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*11011 and 1129 at exon 2 except for a single nucleotide substitution at codon 37, changing the codon from Tyr (DRB1*11011) or Ser (DRB1*1129) to Phe.