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Updated: Aug 4, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Correlation between silver-stained nucleolar organizer region area and cell cycle time
V Canet1, M P Montmasson, Y Usson
1Laboratoire TIMC-IMAG, Institut Albert Bonniot, Université Joseph Fourier Grenoble I, La Tronche, France. Veronique.Canet@imag.fr
Background:
The relationship between the population doubling time and the quantity of silver-stained nucleolar organizer region (AgNOR) interphase proteins was studied in cell culture at three different temperatures used to modulate the cell cycle duration.
Methods:
After MIB 1 and AgNOR combined staining, the quantity of AgNOR proteins was measured in cycling cells by image cytometry.
Results:
Among the several parameters calculated, the AgNOR relative area showed a strong correlation with the changes of the population doubling time induced by different temperatures.
Conclusions:
The results support the hypothesis that the cell cycle time and the size of the ribogenesis machinery are coregulated and that measurements of AgNORs can thus be used as a static evaluation of the cell cycle duration in arbitrary units.
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