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Updated: Aug 18, 2026

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Structure and function of the yeast vacuole and its role in autophagy
1University of Stuttgart, Institute of Biochemistry, Pfaffenwaldring 55, 70569 Stuttgart. thumm@po.uni-stuttgart.de
Abstract:
The vacuole of the yeast Saccharomyces cerevisiae plays an important role in pH- and ion-homeostasis, and is used as a storage compartment for ions. Another important function of the vacuole, especially during nutrient limitation, is the bulk degradation of proteins and even whole organelles. To carry these proteins into the vacuolar lumen, sophisticated transport pathways have evolved. In this review, starvation-induced autophagy and its relationship to the specific cytoplasm to vacuole targeting (cvt-) pathway of proaminopeptidase I is discussed. A further topic is the specific vacuolar uptake and degradation of peroxisomes in Pichia pastoris cells via micro- and macroautophagy.
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