Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput SNP genotyping with the Masscode system.

M Kokoris1, K Dix, K Moynihan

  • 1QIAGEN Genomics, Inc, 1725 220th St. SE, Ste 104, Bothell, WA 98021, USA. m.kokoris@qiagengenomics.com

Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
|February 15, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interstitial Granulomatous Drug Reaction to Ustekinumab.

Case reports in dermatological medicine·2022
Same author

Can we use robotic surgery for the treatment of pelvic recurrence and locally advanced tumors in gynecological surgery?

Journal of gynecology obstetrics and human reproduction·2018
Same author

Do optic nerve head and visual field parameters in patients with obstructive sleep apnea syndrome differ from those in control individuals?

Klinische Monatsblatter fur Augenheilkunde·2014
Same author

Ageing and yield behaviour in model soft colloidal glasses.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2009
Same author

Sleepiness and vigilance tests.

Swiss medical weekly·2009
Same author

Awareness of diabetes complications in an Irish population.

Irish journal of medical science·2009
Same journal

Summary of recent deal activity.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

Diagnostic conferences : january-june 2006.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2013
Same journal

Real-time PCR analysis of trinucleotide repeat allele expansions in the androgen receptor gene.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
Same journal

Prevalence of four virulence genes in Campylobacter jejuni determined by PCR and sequence analysis.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
Same journal

Diagnostic proceeding in Silver-Russell syndrome.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology·2006
See all related articles

QIAGEN

Area of Science:

  • Molecular Biology
  • Genetics
  • Analytical Chemistry

Background:

  • DNA labeling and detection are crucial for genetic analysis.
  • High-throughput genotyping requires efficient and accurate methods.

Purpose of the Study:

  • To describe the application of the Masscode tagging system for high-throughput single-nucleotide polymorphism (SNP) genotyping.
  • To evaluate the Masscode system's performance in SNP discrimination assays.

Main Methods:

  • Utilizing a small-molecular-weight tag covalently attached to DNA oligonucleotides via a photocleavable linker.
  • Employing tagged oligonucleotides as primers in allele-specific PCR assays.
  • Differentiating allele-specific amplicons by Masscode tag assignments and analyzing cleaved tags using mass spectrometry.

Related Experiment Videos

Main Results:

  • The Masscode system enables SNP genotyping through allele-specific PCR and mass spectrometry detection.
  • The system achieves a lower limit of detection in the femtomolar range.
  • Currently supports multiplexing of 30 Masscode tags for over 40,000 daily SNP genotyping measurements.

Conclusions:

  • The Masscode tagging system offers a sensitive and scalable solution for high-throughput SNP genotyping.
  • Future developments aim to increase multiplexing capabilities to several hundred tags.