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Updated: Jul 15, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Automated mass spectrometry: a revolutionary technology for clinical diagnostics
1Sequenom, Inc, 11555 Sorrento Valley Rd., San Diego, CA 92121, USA. jleushner@sequenom.com
Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology
|February 15, 2001
Summary
The MassArray platform offers an automated, cost-effective solution for high-throughput genetic analysis. It integrates advanced technologies for accurate molecular diagnostics, pharmacogenomics, and agricultural applications, improving efficiency and reducing labor.
Area of Science:
- Biotechnology
- Genomics
- Molecular Diagnostics
Background:
- Accurate and cost-effective analytic platforms are crucial for diagnostic analyses and functional genomics studies involving large sample volumes.
- The MassArray platform has been validated for high-throughput diagnostic analyses, combining miniaturized arrays with enzymatic procedures and MALDI-TOF mass spectrometry.
Purpose of the Study:
- To introduce and validate the MassArray platform for high-throughput diagnostic analyses.
- To highlight the platform's capabilities in various applications including molecular diagnostics, pharmacogenomics, and agriculture.
Main Methods:
- Utilizes miniaturized, two-dimensional chip arrays with high-fidelity enzymatic procedures.
- Employs matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for analysis.
- Features nanoliter sample dispensing in high-density formats (384 or greater) for improved throughput and reduced costs.
- Incorporates automation from assay design to data analysis, minimizing labor.
Main Results:
- The platform enables high-throughput analysis with improved efficiency and reduced costs.
- Automation significantly reduces the labor involved in assay development and implementation.
- The system supports diverse applications, including molecular diagnosis of genetic diseases and infectious agents, pharmacogenomics, paternity testing, and agricultural marker-assisted breeding.
- Capability for genotype/phenotype testing on a single platform by analyzing proteins using MALDI-TOF mass spectrometry.
Conclusions:
- The MassArray platform provides a validated, automated, and cost-effective solution for high-throughput genetic and molecular analyses.
- Its versatility supports a wide range of applications in diagnostics, personalized medicine, and agriculture.
- The integration of MALDI-TOF mass spectrometry allows for comprehensive genotype/phenotype testing on a single platform.
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