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Updated: Oct 9, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
Published on: July 17, 2020
Control of cell proliferation via transduction of sPLA(2)-I activity and possible PPAR activation at the nuclear
O Specty1, J F Pageaux, M Dauça
1INSERM U352, Laboratoire de Biochimie et Pharmacologie, INSA-Lyon, 20 Avenue A. Einstein, 69621 Villeurbanne Cedex, France.
Abstract:
Pancreatic phospholipase A2 (PLA(2)-I) stimulates U(III) cells proliferation, a rat uterine cell line, after binding to membrane receptors, internalization and translocation. Here, we demonstrate that during these steps of internalization, PLA(2)-I retains its hydrolytic activity and thus could exert its proliferative effect via nuclear phospholipids hydrolysis. Since fatty acids and eicosanoids released by such activity are known to be ligands of PPAR, we study the expression of these nuclear receptors and demonstrate that, in the experimental conditions where PLA(2)-I stimulates U(III) cells proliferation, PLA(2)-I also regulates PPAR expression indicating a possible mechanism of its proliferative effect.
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