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Cytogenetic analysis of cryopreserved bone marrow cells
A G Bosga-Bouwer1, D Hendriks, E Vellenga
1Department of Medical Genetics, University of Groningen, Ant. Deusinglaan 4, 9713 AW, Groningen, The Netherlands. a.g.bosga-bouwer@medgen.azg.nl
Abstract:
We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells.