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Effects of enamel matrix derivative on gene expression of primary osteoblasts
J Jiang1, A F Fouad, K E Safavi
1Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA.
Objective:
The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts.
Study Design:
Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified Eagle medium as negative control, modified Eagle medium with 25 microg/mL EMD, modified Eagle medium with 100 microg/mL EMD, and modified Eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis.
Result:
EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I.
Conclusion:
These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.
