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Related Experiment Videos

Acapsular Pasteurella multocida B:2 can stimulate protective immunity against pasteurellosis.

J D Boyce1, B Adler

  • 1Bacterial Pathogenesis Research Group, Department of Microbiology, School of Biomedical Sciences, Monash University, Victoria, 3800, Australia.

Infection and Immunity
|February 17, 2001
PubMed
Summary

High doses of a Pasteurella multocida cexA mutant (PBA875) vaccine candidate provided protection against wild-type challenge. An acapsular strain (AL18) also conferred protection when live, but not when killed.

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Area of Science:

  • Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Pasteurella multocida is an important animal pathogen.
  • Capsule export is crucial for P. multocida virulence.
  • Previous studies identified a cexA mutant (PBA875) with impaired capsule export and reduced virulence.

Purpose of the Study:

  • To evaluate the protective efficacy of a P. multocida cexA mutant (PBA875) as a vaccine.
  • To construct and characterize a genetically defined acapsular P. multocida strain (AL18).
  • To assess the immunoprotective potential of the acapsular strain.

Main Methods:

  • Immunization of mice with different doses of PBA875.
  • Construction of an acapsular P. multocida strain (AL18) by inactivating the bcbH gene.

Related Experiment Videos

  • Assessment of polysaccharide production using immunofluorescence and Western immunoblot.
  • Challenge studies with wild-type P. multocida in immunized mice.

    • Main Results:

    • High doses of PBA875 conferred significant protection against wild-type P. multocida challenge.
    • The acapsular strain AL18 failed to produce detectable polysaccharide.
    • Live AL18 immunization provided significant protection against wild-type challenge.
    • Killed wild-type or killed AL18 failed to confer protection.

    Conclusions:

    • Vaccination with high doses of the P. multocida cexA mutant (PBA875) can induce protective immunity.
    • A live, acapsular P. multocida strain (AL18) is a promising vaccine candidate.
    • The protective immunity elicited by live AL18 suggests the importance of live bacterial components beyond the capsule.