Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BglII and MunI: what a difference a base makes.

C M Lukacs1, A K Aggarwal

  • 1Hoffman-La Roche Inc, 340 Kingsland Street, Nutley, NJ 07110, USA. Christine.lukacs@roche.com

Current Opinion in Structural Biology
|February 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Managing bone defects in primary total knee arthroplasty: options and current trends.

Musculoskeletal surgery·2020
Same author

Tele-evidence: A videoconferencing tool as a viable alternative to physical appearance of doctors for the judicial summons.

Journal of postgraduate medicine·2018
Same author

Therapeutic Evaluation of Topical Retinoic Acid in Acne Vulgaris.

Indian journal of dermatology, venereology and leprology·2017
Same author

"Pine tree" appearance on <sup>18</sup>F-FDG PET/CT MIP image in spinal tuberculosis.

Revista espanola de medicina nuclear e imagen molecular·2016
Same author

Orbital proptosis in a young immunocompetent female patient.

Medical journal, Armed Forces India·2015
Same author

Collapse pressure analysis of transversely isotropic thick-walled cylinder using Lebesgue strain measure and transition theory.

TheScientificWorldJournal·2014
Same journal

Progress toward linking single-molecule behavior and condensate material properties.

Current opinion in structural biology·2026
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
See all related articles

Restriction endonucleases maintain their DNA-binding specificity despite sequence changes. Structural comparisons of BglII and MunI complexes reveal insights into this enzyme immutability.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Enzymology

Background:

  • Restriction endonucleases are enzymes that cleave DNA at specific recognition sites.
  • These enzymes are crucial tools in molecular biology and genetic engineering.
  • Understanding their DNA-binding specificity is key to their application.

Purpose of the Study:

  • To investigate the structural basis for the conserved DNA-binding specificities of restriction endonucleases.
  • To compare the structures of BglII and MunI endonucleases bound to their respective DNA sites.
  • To elucidate the factors contributing to the immutability of restriction endonuclease recognition.

Main Methods:

  • X-ray crystallography was used to determine the structures of BglII-DNA and MunI-DNA complexes.

Related Experiment Videos

  • Comparative structural analysis of the determined complexes.
  • Bioinformatic analysis of enzyme-DNA interactions.

    • Main Results:

    • Structures of BglII and MunI endonucleases bound to their palindromic DNA recognition sites were determined.
    • These sites differ only in their outer base pairs from related enzyme recognition sequences (BamHI and EcoRI).
    • The comparison revealed significant structural differences and similarities, offering insights into specificity.

    Conclusions:

    • The structural comparison provides a basis for understanding why restriction endonucleases are resistant to alterations in their DNA-binding specificities.
    • The findings contribute to the fundamental knowledge of protein-DNA interactions.
    • This research aids in the rational design of engineered restriction enzymes.