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Regulating genes with electromagnetic response elements.

H Lin1, M Blank, K Rossol-Haseroth

  • 1Department of Pathology, Columbia University Health Sciences, New York, NY 10032, USA.

Journal of Cellular Biochemistry
|February 17, 2001
PubMed
Summary
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Specific DNA sequences, termed electromagnetic field response elements (EMREs), are essential for regulating gene expression in response to electromagnetic fields. Introducing these EMREs can control gene activity, offering potential for gene therapy applications.

Area of Science:

  • Molecular Biology
  • Biophysics

Background:

  • Electromagnetic (EM) fields can influence biological processes, including gene expression.
  • Specific DNA sequences within gene promoters are known to regulate gene induction.

Purpose of the Study:

  • To identify and characterize DNA elements responsible for EM field-induced gene expression.
  • To investigate the potential of these elements as regulatory switches for gene therapy.

Main Methods:

  • Deletion analysis of c-myc and HSP70 promoters to identify essential regulatory regions.
  • Introduction of identified response elements (EMREs) into reporter gene constructs (CAT and luciferase).
  • Transfection of constructs into HeLa cells and exposure to controlled 8 microT 60 Hz EM fields.

Main Results:

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  • A 900 bp segment of the c-myc promoter and a 70 bp region of the HSP70 promoter, each containing nCTCTn sequences, were found to be critical for EM field-induced expression.
  • These nCTCTn sequences, termed EMREs, were shown to mediate the response to EM fields.
  • Reporter constructs containing EMREs exhibited significantly increased CAT and luciferase activity upon EM field exposure, while constructs lacking EMREs did not.

Conclusions:

  • The nCTCTn sequences function as electromagnetic field response elements (EMREs).
  • EMREs can confer responsiveness to EM fields in otherwise non-responsive reporter gene constructs.
  • EMREs hold promise as controllable switches for regulating gene expression in gene therapy applications.