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In vitro development of resistance to six quinolones in Streptococcus pneumoniae, Streptococcus pyogenes, and
1Institute for Medical Microbiology and Virology, Heinrich-Heine Universität Düsseldorf, Düsseldorf, Germany.
Abstract:
Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus isolates were exposed to subinhibitory MICs of ciprofloxacin, sparfloxacin, gatifloxacin, moxifloxacin, clinafloxacin, and gemifloxacin during a 10-day period. Subculturing led to resistance development, regardless of the initial potencies of the quinolones. None of the quinolones was associated with a significantly slower rate of resistance development.
Insights
Development of bacterial resistance to fluoroquinolones like ciprofloxacin is rapid. This study found that none of the tested fluoroquinolones significantly slowed resistance development in common bacteria like Streptococcus pneumoniae.
Area of Science:
- Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Fluoroquinolones are critical antibiotics for treating bacterial infections.
- Emergence of antibiotic resistance is a major global health threat.
- Understanding resistance development mechanisms is crucial for effective treatment strategies.
Purpose of the Study:
- To investigate the rate of resistance development in key bacterial pathogens exposed to various fluoroquinolones.
- To compare the efficacy of different fluoroquinolones in preventing or slowing resistance.
- To assess the impact of subinhibitory concentrations on resistance emergence.
Main Methods:
- Exposure of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus isolates to subinhibitory Minimum Inhibitory Concentrations (MICs) of six fluoroquinolones.
- Daily subculturing over a 10-day period to monitor resistance.
- Quantitative assessment of resistance development rates.
Main Results:
- Rapid development of resistance was observed across all tested bacterial isolates.
- Resistance emerged irrespective of the initial potency of the fluoroquinolone used.
- No significant difference was found in the rate of resistance development among the tested fluoroquinolones.
Conclusions:
- Subinhibitory concentrations of fluoroquinolones promote rapid resistance development in common bacterial pathogens.
- Current fluoroquinolones do not appear to offer a significant advantage in delaying resistance.
- Further research is needed to develop strategies to mitigate fluoroquinolone resistance.