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Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer
C Torres Viera1, S Tsiodras, H S Gold
1Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Antimicrobial Agents and Chemotherapy
|February 22, 2001
Abstract:
We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.