[Effect of increased pressure and changed gaseous media on biological properties of microorganisms]

Bacterial contamination of diving outfit and inner surfaces of pressure complex increases at the expense of expansion of gram-negative microflora during long exploitation of undersea complexes. Gram-negative bacteria were hypothesized to effectively adapt to hyperbaric conditions. We investigated the effect of hyperbaric conditions and changed gaseous environment on the cultural and morphological characteristics of colonies, growth rate and time of generation of test cultures (P. aeruginosa ATCC 10145, E. coli Tg1, and Bac. subtilis GB2036). Phenotypically modified clones were selected for subsequent analysis of changes at a genetic level. Experiments revealed no essential changes in the studied properties under the effect of extreme conditions.