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Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

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A diffusion model for the coordination of DNA replication in Schizosaccharomyces pombe.

Scientific reports·2016
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Licensing of DNA replication, cancer, pluripotency and differentiation: an interlinked world?

Seminars in cell & developmental biology·2014
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Isolation and characterization of the amino-acid pools located within the cytoplasm and vacuoles of Candida utilis.

Planta·2014
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High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

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Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing.

Proceedings of the National Academy of Sciences of the United States of America·2009
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Stochastic hybrid modeling of DNA replication across a complete genome.

Proceedings of the National Academy of Sciences of the United States of America·2008

Related Experiment Video

Updated: Jul 16, 2026

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Cell cycle. License withheld--geminin blocks DNA replication.

Z Lygerou1, P Nurse

  • 1Laboratory of General Biology, School of Medicine, University of Patras, 26110 Rio, Patras, Greece. z_lygerou@yahoo.com

Science (New York, N.Y.)
|February 24, 2001
PubMed
Summary

Cell division requires genome replication licensing to occur only once per cell cycle. Geminin protein binding to Cdt1 prevents licensing factor access to chromatin, thereby blocking S phase entry.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cell cycle progression relies on precise regulation of DNA replication.
  • Genome duplication must occur only once per cell cycle to maintain genomic stability.

Discussion:

  • Geminin protein acts as a crucial inhibitor of DNA replication licensing.
  • The interaction between Geminin and Cdt1 is key to preventing re-replication.
  • This mechanism ensures the fidelity of DNA replication during cell division.

Key Insights:

  • Geminin binding to Cdt1 directly blocks licensing factors from accessing chromatin.
  • This inhibition effectively prevents the initiation of S phase, enforcing a single round of DNA replication.
  • Understanding this regulatory step is vital for comprehending cell cycle control.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Related Experiment Videos

Last Updated: Jul 16, 2026

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
11:23

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

Published on: May 22, 2012

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Outlook:

  • Further research into the Geminin-Cdt1 complex could reveal new therapeutic targets for cancer.
  • Investigating the precise structural interactions may lead to novel strategies for controlling cell proliferation.
  • This regulatory pathway is fundamental to eukaryotic cell division and genome integrity.