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[Genetic instability of microsatellites Mfd41 and DCC in the evolution of chronic myelogenous leukemia]
Objective:
To explore the relationship between the genetic instability of microsatellite and the evolution of chronic myelogenous leukemia(CML).
Methods:
The loss of heterozygosity(LOH) and the microsatellite instability(MSI) of two polymorphic microsatellite markers, DCC and Mfd41, which located on chromosome 18q and 17p respectively, were assayed by standard PCR-silver staining analysis in bone marrow cells from 17 CML patients progressing from chronic phase to accelerated phase or blast crisis.
Results:
LOH or MSI of the two microsatellites were demonstrated in 8/17 (47.5%) patients in accelerated/blastic phases. For DCC, genetic instability was revealed in 2 of 9 patients in accelerated phase and in 4 of 8 in blast crisis. For Mfd41, genetic instability was revealed in 1 of 9 patients in accelerated and in 1 of 8 in blast crisis.
Conclusion:
Genetic instability of DCC and Mfd41 may play a role in the evolution of CML.