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Related Experiment Videos

Recent developments in electrokinetically driven analysis on microfabricated devices.

G J Bruin1

  • 1Novartis Pharma AG, Drug Metabolism & Pharmacokinetics, Basel, Switzerland. gerardus_j_m.bruin@pharma.novartis.com

Electrophoresis
|February 24, 2001
PubMed
Summary

Microfluidic separation devices, driven by electrokinetic flow, are rapidly advancing. Recent progress shows integrated lab-on-a-chip systems performing complex analytical tasks with improved designs and detection methods.

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Microfluidic devices offer miniaturized platforms for various analytical applications.
  • Electrokinetic flow is a key driving force in microfluidic separation.
  • Lab-on-a-chip (LOC) technology aims to integrate multiple laboratory functions onto a single chip.

Purpose of the Study:

  • To provide an overview of recent advancements in electrokinetically driven microfluidic separation devices.
  • To highlight trends and progress in microchip-based separation systems over the last three years.
  • To discuss the integration of analytical tasks and novel materials/detection methods in microfluidics.

Main Methods:

  • Review of recent literature on microfluidic separation devices.

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  • Analysis of microchip layouts, sample handling strategies, and capillary array systems.
  • Examination of functional elements for on-chip preconcentration, filtering, DNA amplification, and detection.
  • Discussion of alternative materials (PDMS, PMMA) and detection techniques (MS, electrochemical).

    • Main Results:

    • Significant progress in microchip layout optimization, multi-sample handling, and capillary array systems.
    • Emerging integration of diverse analytical functions (preconcentration, filtering, amplification, detection) on single microchips.
    • Successful transfer of capillary electrophoresis (CE) separation modes to microchip platforms.
    • Increased use of polymers like PDMS and PMMA for chip fabrication and adoption of MS and electrochemical detection.

    Conclusions:

    • Microfluidic separation devices are rapidly evolving, moving towards fully integrated lab-on-a-chip systems.
    • The field shows substantial progress in device design, functional integration, and material/detection diversity.
    • Microchip CE is a mature technology with a broad range of applications, indicating a promising future for microfluidic separations.