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Updated: Oct 1, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Surfactant cloud point extraction and preconcentration of organic compounds prior to chromatography and capillary
R Carabias-Martínez1, E Rodríguez-Gonzalo, B Moreno-Cordero
1Department of Analytical Chemistry, Nutrition and Food Sciences, School of Chemistry, University of Salamanca, Spain. rcm@gugu.usal.es
Abstract:
The use of preconcentration steps based on phase separation by the cloud point technique offers a convenient alternative to more conventional extraction systems. It has been used successfully for the preconcentration of species of widely differing character and nature, such as metal ions, proteins and other biomaterials, or organic compounds of strongly differing polarity. Here we address the most recent analytical applications of this methodology when used as an isolation and trace enrichment step prior to the analysis of organic compounds (polycyclic aromatic hydrocarbons, polychlorinated compounds, pesticides, phenolic derivatives, aromatic amines, vitamins, etc.) via liquid and gas chromatography or capillary electrophoresis.
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