Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Osteoprogenitor cells within skeletal muscle.

P Bosch1, D S Musgrave, J Y Lee

  • 1Department of Orthopaedic Surgery, Musculoskeletal Research Center, University of Pittsburgh and Children's Hospital of Pittsburgh, Pennsylvania 15261, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|February 24, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Therapeutic drug monitoring and TB treatment outcomes in patients with diabetes mellitus.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Fields on fire: Alternatives to crop residue burning in India.

Science (New York, N.Y.)·2019
Same author

Non-adherence and drug-related interruptions are risk factors for delays in completion of treatment for tuberculosis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2013
Same author

Chronic lung disease and HIV infection are risk factors for recurrent tuberculosis in a low-incidence setting.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2011
Same author

Regeneration of dystrophin-expressing myocytes in the mdx heart by skeletal muscle stem cells.

Gene therapy·2005
Same author

Muscle cell-mediated gene delivery to the rotator cuff.

Tissue engineering·2003

Osteoprogenitor cells, crucial for bone formation, reside within skeletal muscle tissue. This study found muscle-derived cells can differentiate into bone cells, suggesting muscle as a potential source for these cells.

Area of Science:

  • Skeletal biology
  • Regenerative medicine
  • Cell biology

Background:

  • Ectopic bone formation in skeletal muscle is common, but the origin of osteoprogenitor cells is unclear.
  • Identifying the source of these cells is critical for understanding bone development and regeneration.

Purpose of the Study:

  • To investigate whether skeletal muscle tissue contains osteoprogenitor cells capable of differentiating into bone.
  • To explore muscle as an alternative source for harvesting osteoprogenitor cells.

Main Methods:

  • Isolated subpopulations of adult mouse skeletal muscle cells.
  • Assessed alkaline phosphatase activity after exposure to bone morphogenetic protein-2 in vitro.
  • Injected genetically modified muscle cells into severe combined immunodeficient (SCID) mice to track their fate.

Related Experiment Videos

Main Results:

  • A subpopulation of muscle cells showed osteogenic differentiation potential in vitro.
  • Injected muscle-derived cells participated in ectopic bone formation in vivo.
  • LacZ-positive cells (indicating muscle origin) were found within newly formed bone, co-localized with osteocalcin.

Conclusions:

  • Skeletal muscle harbors cells with osteoprogenitor potential.
  • These muscle-derived cells can differentiate into osteoblasts and osteocytes.
  • Skeletal muscle represents a potential alternative source for osteoprogenitor cells, distinct from bone marrow.