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Molecular cloning and characterization of a cDNA for a rice Sec31p homolog

T Kato1, A Ishikawa, T Asahi

  • 1Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Japan.

Bioscience, Biotechnology, and Biochemistry
|February 24, 2001
PubMed

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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...