Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zonal differentiation in the rat adrenal cortex.

E Whitworth1, G P Vinson

  • 1Molecular and Cellular Biology Section, Queen Mary & Westfield College, University of London.

Endocrine Research
|February 24, 2001
PubMed
Summary

Adrenal gland zonation in vitro is maintained intrinsically. Stimulants like potassium, ACTH, and angiotensin II did not alter this inherent zonal structure but affected steroid output.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-cancer actions of a recombinant antibody (R6313/G2) against the angiotensin II AT1 receptor.

Endocrine-related cancer·2008
Same author

The role of angiotensin II in the regulation of breast cancer cell adhesion and invasion.

Endocrine-related cancer·2006
Same author

Regulation of hepatic steroid receptors and enzymes by the 3beta-hydroxysteroid dehydrogenase inhibitor trilostane.

The Journal of steroid biochemistry and molecular biology·2006
Same author

Localisation of renin-angiotensin system (RAS) components in breast.

British journal of cancer·2006
Same author

Distribution of extracellular signal-regulated protein kinases 1 and 2 in the rat adrenal and their activation by angiotensin II.

The Journal of endocrinology·2005
Same author

ACTH modulates ERK phosphorylation in the adrenal gland in a time-dependent manner.

Endocrine research·2005

Area of Science:

  • Endocrinology
  • Cell Biology
  • Adrenal Gland Physiology

Background:

  • Adrenocortical zonation, crucial for hormone production, is poorly understood.
  • Previous studies showed in vivo development of functional zonation in rat adrenal autotransplants.

Purpose of the Study:

  • To investigate the in vitro maintenance of adrenocortical zonation.
  • To determine the effects of potassium, ACTH, and angiotensin II on zonal structure and steroidogenesis.

Main Methods:

  • Cultured rat adrenal capsular preparations (zona glomerulosa/fasciculata) in Eagles MEM.
  • Assessed zonal differentiation using immunocytochemistry for Pref-1 (zona glomerulosa) and IZA (zona fasciculata/reticularis).
  • Measured aldosterone and corticosterone output under various stimulant conditions (potassium, ACTH, angiotensin II, losartan).

Main Results:

  • Adrenal preparations maintained intrinsic zonal arrangement (zona glomerulosa/fasciculata) for 14 days without significant proliferation.
  • Stimulants (potassium, ACTH, angiotensin II) and losartan did not affect the inherent capacity to maintain zonation.
  • Steroid output was complexly affected: aldosterone output was stimulated by AII or high K+, while corticosterone increased autonomously then declined.

Conclusions:

  • The intrinsic property of the adrenal gland to express zona glomerulosa and zona fasciculata phenotypes is maintained in vitro.
  • While zonal structure is stable, steroidogenic capacity is specifically and complexly modulated by external factors.

Related Experiment Videos