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Updated: Aug 9, 2026
09:40
Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning to reproduce desired genotypes
M E Westhusin1, C R Long, T Shin
1Departments of Veterinary Physiology and Pharmacology, Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA. MWESTHUSIN@cvm.tamu.edu
Theriogenology
|February 24, 2001
Summary
Animal cloning using somatic cell nuclear transfer has successfully produced various species, though efficiency remains low. Research suggests broad applicability for reproducing desired animal genotypes, pending further investment.
Area of Science:
- Reproductive Biology
- Genetics
- Animal Science
Background:
- Somatic cell nuclear transfer (SCNT) has enabled the cloning of multiple animal species, including sheep, cattle, goats, pigs, and mice.
- Despite successes, animal cloning remains inefficient, with live offspring resulting from less than 10% of transferred cloned embryos.
Purpose of the Study:
- To explore the potential and influencing factors of reproducing specific animal genotypes via cloning.
- To assess the broad applicability of cloning technology across diverse animal species.
Main Methods:
- Somatic cell nuclear transplantation into recipient ova.
- Evaluation of variables influencing cloning success, such as species, donor cell type, and nuclear transfer techniques.
Main Results:
- Successful cloning demonstrated across various species and by multiple laboratories.
- Identified key variables impacting the efficiency of reproducing specific genotypes through cloning.
Conclusions:
- Cloning technology shows promise for reproducing desired genotypes, including genetically modified lines, prize livestock, pets, and endangered species.
- Current data indicates cloning is likely applicable to a wide range of animals, not limited to a few species.
- Further research investment is crucial to fully realize the potential of cloning for genotype reproduction.
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