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Published on: September 11, 2015
Adhesion of human osteoblasts on root-end filling materials
1Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center 06030-1715, USA.
Journal of Endodontics
|February 24, 2001
Summary
Human osteoblasts show better adhesion to mineral trioxide aggregate (MTA) and composite resins compared to IRM and amalgam. This suggests MTA and composite are more favorable root-end filling materials for bone cell growth.
Area of Science:
- Biomaterials Science
- Cell Biology
- Dental Materials
Background:
- Root-end filling materials are crucial for endodontic treatment success.
- Understanding osteoblast adhesion to these materials is vital for bone regeneration.
- Mineral trioxide aggregate (MTA), IRM, composite, and amalgam are commonly used materials.
Purpose of the Study:
- To evaluate and compare the adhesion and spreading of human osteoblasts on four different root-end filling materials.
- To determine the biocompatibility of MTA, IRM, composite, and amalgam concerning osteoblast interaction.
Main Methods:
- Human osteoblasts were cultured on disk-shaped samples of MTA, IRM, composite, and amalgam.
- Cell adhesion and morphology were examined using scanning electron microscopy after 1 day of culture.
Main Results:
- Osteoblasts readily attached and spread on mineral trioxide aggregate (MTA) and composite, forming a monolayer.
- Attachment was observed on amalgam, but with limited cell spreading.
- Osteoblasts remained rounded with no spreading on IRM, indicating poor adhesion.
Conclusions:
- Mineral trioxide aggregate (MTA) and composite resin demonstrate superior osteoblast adhesion and spreading compared to IRM and amalgam.
- MTA and composite are favorable root-end filling materials for promoting osteoblast response and potential osseointegration.
