Measurement of endodontic file lengths: a density profile plot analysis

The purpose of this study was to evaluate measurements of endodontic files of known length and diameter using a density profile plot analysis of digital images. Ten single-rooted teeth with relatively straight roots in cadaver specimens were used. The crowns of the teeth were removed and a rectangular orthodontic wire, 5.13 mm in length, was placed horizontally on the occlusal surface to serve as a calibration reference point. The #8, #10, #15, and #20 FlexOFiles were measured to the nearest 0.5 mm and then placed to four working lengths that terminated within the apical third of each root. A GE X-ray unit and a Schick CDR #2 sensor were used to digitally acquire 160 images. The digital images were placed in random order and an independent, blinded investigator determined the file length using a density profile plot analysis. The measurements generated by the histogram analysis (experimental) were compared with the original clinical measurements. The paired t test, intraclass correlation coefficient, and the Bradley-Blackwood test were used to assess reliability. The results revealed that the means of the experimental measurements of all file sizes were within 0.5 mm of the known lengths and were always shorter than the known lengths. Also, the larger the file size the less deviation from the known lengths: #20, -0.16 mm (p = 0.0001); #15, -0.21 mm (p = 0.0001); #10, -0.34 mm (p = 0.0001); and #8, -0.45 mm (p = 0.0001). This study demonstrated that the density profile plot analysis might be a useful adjunct for the measurement of endodontic file lengths on a digital image.