Related Experiment Videos
Synthesis of N-labeled peptidyl AMP
H Narita1, T Moriguchi, K Seio
1Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
Nucleosides, Nucleotides & Nucleic Acids
|February 24, 2001
Abstract:
This paper deals with the synthesis of a new type of N-labeled peptidyl AMP, which would be used as a good substrate for analysis of the peptidyl transfer reaction on ribosome and for co-crystallization with ribosome. 4-(Dimethylamino)azobenzene-4'-sulfonyl (Dabsyl) was selected as the labeling group. (N-Dabsylglycyl)-L-leucyl AMP was synthesized from glycyl-L-leucine via a three-step procedure.