Related Experiment Video

Updated: Aug 14, 2026

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses
06:13

Handling of the Cotton Rat in Studies for the Pre-clinical Evaluation of Oncolytic Viruses

Published on: November 24, 2014

A conditionally replicative adenovirus with enhanced infectivity shows improved oncolytic potency

K Suzuki1, J Fueyo, V Krasnykh

  • 1Gene Therapy Center, University of Alabama at Birmingham, 35294-3300, USA.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|February 24, 2001
PubMed

Insights

Engineered adenoviruses overcome tumor cell receptor limitations for enhanced cancer treatment. Modified viruses show improved tumor cell infection, replication, and lysis, leading to greater oncolytic potency in vivo.

Area of Science:

  • Oncolytic virotherapy
  • Adenovirus engineering
  • Cancer treatment

Background:

  • The Coxsackievirus and adenovirus receptor (CAR) is crucial for adenovirus entry into cells.
  • Limited CAR expression on certain tumors restricts the effectiveness of current oncolytic adenoviruses.
  • Conditionally replicative adenoviruses (CRAds) are engineered to selectively target and destroy cancer cells.

Purpose of the Study:

  • To enhance the oncolytic potency of CRAds by enabling CAR-independent tumor cell infection.
  • To investigate the efficacy of genetically modified adenoviruses with altered fiber knobs for improved cancer treatment.

Main Methods:

  • Genetic modification of the adenovirus fiber knob to facilitate CAR-independent infection.
  • Utilized an E1A-defective CRAd backbone for tumor-specific replication.
  • Evaluated viral replication, tumor cell infection, and lysis in vitro and in vivo.

Main Results:

  • The fiber-modified CRAd demonstrated enhanced infectivity and replication in tumor cells.
  • Significant lysis of tumor cells was observed in vitro with the modified adenovirus.
  • Improved antitumor effects were confirmed in vivo, highlighting the enhanced efficacy of the fiber-modified CRAd.

Conclusions:

  • Combining genomic modifications for tumor selectivity with capsid modifications for enhanced infectivity creates more potent oncolytic adenoviruses.
  • This dual-modification strategy represents a promising approach for developing effective adenoviral cancer therapies.
  • Fiber modification of CRAds offers a viable strategy to overcome CAR-dependent entry limitations in cancer treatment.

More Related Videos

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Related Concept Videos

Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...