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Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage can Stall the Cell Cycle02:36

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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

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Related Experiment Video

Updated: May 11, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression.

L Y Marmorstein1, A V Kinev, G K Chan

  • 1The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.

Cell
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Researchers identified a BRCA2-Associated Factor 35 (BRAF35) in a BRCA2 complex. This BRAF35 protein associates with condensed chromatin and may regulate cell cycle progression in breast cancer susceptibility.

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Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Related Experiment Videos

Last Updated: May 11, 2026

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1
08:53

Identifying the Effects of BRCA1 Mutations on Homologous Recombination using Cells that Express Endogenous Wild-type BRCA1

Published on: February 18, 2011

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Germline mutations in the human BRCA2 gene are linked to increased breast cancer risk.
  • The precise function of the BRCA2 protein is not fully understood.
  • BRCA2-deficient mouse cells exhibit chromosomal abnormalities.

Purpose of the Study:

  • To investigate the function of the BRCA2 protein.
  • To identify components of the BRCA2 complex.
  • To explore the role of BRCA2 in cellular processes.

Main Methods:

  • Isolation and characterization of a large BRCA2-containing complex.
  • Identification and analysis of a DNA-binding component, BRAF35.
  • Expression analysis of BRAF35 mRNA in mouse embryos.
  • Immunofluorescence staining to assess BRAF35/BRCA2 localization.
  • Antibody microinjection to study functional roles.

Main Results:

  • A 2 MDa BRCA2 complex was isolated, containing a DNA-binding protein named BRAF35.
  • BRAF35 possesses an HMG domain for DNA binding and a kinesin-like coiled-coil domain.
  • BRAF35 mRNA expression mirrors BRCA2 expression, being highest in proliferating embryonic tissues.
  • The BRAF35/BRCA2 complex localizes with condensed chromatin during mitosis.
  • Antibody microinjection suggests the complex influences cell cycle progression.

Conclusions:

  • BRAF35 is a novel DNA-binding component of the BRCA2 complex.
  • The BRCA2/BRAF35 complex is associated with chromatin and may play a role in cell cycle regulation.
  • Further research into the BRCA2/BRAF35 complex could elucidate mechanisms underlying breast cancer susceptibility.