Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epithelial calcium transporter expression in human duodenum.

N F Barley1, A Howard, D O'Callaghan

  • 1Gastroenterology Section, Imperial College School of Medicine, Hammersmith Campus, London W12 0NN, United Kingdom.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|February 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Observation of Suppressed Charged-Particle Production in Ultrarelativistic Oxygen-Oxygen Collisions.

Physical review letters·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026
Same journal

MYO5B Deficiency Is Associated with Altered MUC13 Localization and DMBT1 Accumulation in Intestinal Epithelial Cells.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same journal

Microbiota dysbiosis and mucosal hyperinnervation contribute to intestinal hyperalgesia in a mouse model of post-infectious irritable bowel syndrome.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same journal

Roux-en-Y gastric bypass alters microbial circadian rhythms with links to metabolic improvement.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same journal

Therapeutic zinc targets dysregulated GC-C signaling and restores ileal defects in a preclinical model of familial diarrheal disease.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same journal

Epithelial fucosylation regulates intestinal stem cell differentiation.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same journal

Claims regarding the fidelity of extracellular electrical recording from gastrointestinal muscles not validated suitably.

American journal of physiology. Gastrointestinal and liver physiology·2026
See all related articles

This study identifies a human calcium channel transcript (ECAC2) primarily in the duodenum. Its expression correlates with other calcium transport genes, suggesting linked regulation despite individual variations not being vitamin D dependent.

Area of Science:

  • Physiology
  • Molecular Biology
  • Gastroenterology

Background:

  • Intestinal and kidney calcium absorption involves epithelial cell transport.
  • Apical calcium influx channels like ECaC and CaT1 have been identified in other species.

Purpose of the Study:

  • To identify and characterize human calcium channel transcripts involved in intestinal calcium absorption.
  • To investigate the relationship between calcium channel gene expression, vitamin D metabolites, and other calcium transport proteins.

Main Methods:

  • Amplification of a partial human cDNA probe (ECAC2) from duodenal tissue.
  • Analysis of transcript expression in various human tissues using Northern blotting.
  • Correlation analysis of ECAC2 transcript levels with vitamin D metabolites and calbindin-D(9k) and PMCA1 expression in duodenal biopsies.

Related Experiment Videos

Main Results:

  • A 3-kb transcript, ECAC2, was predominantly expressed in the human duodenum.
  • ECAC2 expression showed significant positive correlations with calbindin-D(9k) and PMCA1.
  • Individual variations in ECAC2 transcript levels were not significantly correlated with vitamin D metabolites.

Conclusions:

  • Human duodenal calcium absorption involves a specific calcium channel transcript, ECAC2.
  • Expression of genes for apical calcium entry and basolateral extrusion appears tightly linked.
  • This coordinated gene expression may explain variability in calcium absorption.