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MYO5B Deficiency Is Associated with Altered MUC13 Localization and DMBT1 Accumulation in Intestinal Epithelial Cells.

Rachel Stubler1, Charulekha Packirisamy1, Jenna G Cagle1

  • 1Department of Regenerative Medicine & Cell Biology, Medical University of South Carolina, Charleston, SC USA.

American Journal of Physiology. Gastrointestinal and Liver Physiology
|July 1, 2026
PubMed
Summary
This summary is machine-generated.

Myosin 5b (MYO5B) is crucial for delivering protective glycoproteins MUC13 and DMBT1 to the intestinal surface. Its loss causes intracellular buildup and degradation, impacting mucosal defense.

Keywords:
MYO5Bcellular traffickingcolonmucinsmall intestine

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Area of Science:

  • Cell Biology
  • Gastroenterology
  • Molecular Biology

Background:

  • Myosin 5b (MYO5B) is a motor protein vital for apical membrane protein trafficking.
  • MYO5B transports various transporters and channels, but its role in glycoprotein trafficking for mucosal defense is unknown.
  • MUC13 and DMBT1 are critical glycoproteins for epithelial protection and wound healing.

Purpose of the Study:

  • To investigate if MYO5B is required for the apical localization of MUC13 and DMBT1.
  • To determine the impact of MYO5B loss on glycoprotein trafficking in the intestinal epithelium.

Main Methods:

  • Immunostaining of small intestine and colon in MYO5B-knockout mice (neonatal germline and adult inducible).
  • Analysis of intestinal organoids derived from MYO5B-knockout mice.
  • Staining of human intestinal organoids expressing MYO5B-Tail GFP.

Main Results:

  • MYO5B loss led to intracellular accumulation of MUC13 and DMBT1, reducing apical colocalization.
  • MUC13 colocalized with LAMP1 in MYO5B-deficient adult mice, suggesting lysosomal degradation.
  • MUC13 mislocalization was observed in vitro in MYO5B-deficient organoids.
  • MYO5B-Tail GFP associated with MUC13 in human organoids.

Conclusions:

  • MYO5B is essential for the apical delivery of MUC13 and DMBT1 in the intestinal epithelium.
  • Disruption of MYO5B-mediated trafficking may contribute to mucosal dysfunction in MYO5B-related diseases.
  • Targeting this pathway could offer therapeutic strategies for restoring epithelial barrier integrity.