Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.3K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.3K
Histology of the Small Intestine01:27

Histology of the Small Intestine

4.0K
The small intestine exhibits a unique histological structure that significantly enhances its function in digestion and nutrient absorption. These structures include circular folds, villi, and various specialized cells that collectively facilitate the digestion of food.
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
4.0K
Microvilli00:55

Microvilli

9.7K
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
9.7K
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

2.5K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MYO5B Deficiency Is Associated with Altered MUC13 Localization and DMBT1 Accumulation in Intestinal Epithelial Cells.

American journal of physiology. Gastrointestinal and liver physiology·2026
Same author

MUC4-targeted CAR-T Cells Restrain Chemotherapy-resistant Colorectal Cancer.

bioRxiv : the preprint server for biology·2026
Same author

Saffron Alters Microbial Amino Acid Metabolism and Neurotransmitter Production in a Defined Gut Consortium.

Food science & nutrition·2026
Same author

A pilot study of maternal vitamin D status and its association with breast milk and infant gut metabolites.

Physiological reports·2026
Same author

Breaking down barriers: is intestinal mucus degradation by <i>Akkermansia muciniphila</i> beneficial or harmful?

Infection and immunity·2025
Same author

Viroporin activity is necessary for intercellular calcium signals that contribute to viral pathogenesis.

Science advances·2025

Related Experiment Video

Updated: Feb 17, 2026

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging
05:10

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

Published on: September 12, 2025

526

Akkermansia muciniphila Selectively Reshapes Small Intestinal Cell Populations.

Rachel Edens-Valentine1, Kristen A Engevik1, Melinda A Engevik1,2

  • 1Department of Regenerative Medicine and Cell Biology.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|February 16, 2026
PubMed
Summary

Akkermansia muciniphila colonization in mice increased small intestinal tuft cells and altered mucus composition. This suggests A. muciniphila plays a role in gut homeostasis through succinate signaling.

Keywords:
AkkermansiaTuft cellsepitheliumgnotobioticmono-associated

More Related Videos

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
11:34

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Published on: July 26, 2019

9.2K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

73.4K

Related Experiment Videos

Last Updated: Feb 17, 2026

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging
05:10

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging

Published on: September 12, 2025

526
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
11:34

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Published on: July 26, 2019

9.2K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

73.4K

Area of Science:

  • Microbiology
  • Gastroenterology
  • Immunology

Background:

  • The gut microbiome influences intestinal health.
  • Akkermansia muciniphila is a mucin-degrading bacterium with known health benefits.
  • Its specific role in the small intestine is not well understood.

Purpose of the Study:

  • To investigate the direct impact of Akkermansia muciniphila on the small intestine.
  • To explore the mechanisms by which A. muciniphila affects intestinal cells and immune components.

Main Methods:

  • Gnotobiotic mice were colonized with A. muciniphila or a control.
  • Small intestinal tissues were analyzed using fluorescence in situ hybridization and immunofluorescence staining.
  • Metabolomic analysis identified key bacterial products.

Main Results:

  • A. muciniphila successfully colonized the small intestine.
  • Increased epithelial cell proliferation, altered mucus composition (reduced fucose), and elevated tuft cell and ILC2 numbers were observed.
  • Succinate was identified as a potential mediator produced by A. muciniphila.

Conclusions:

  • Akkermansia muciniphila alone can increase tuft cells in the small intestine, likely via succinate.
  • These findings highlight a novel role for A. muciniphila in regulating small intestinal homeostasis and host-microbe interactions.