Fiberoptic biosensors based on chemiluminescent reactions

Area of Science:

• Analytical Chemistry
• Biotechnology
• Sensor Technology

Background:

• Chemiluminescence of luminol with hydrogen peroxide (H2O2) is a basis for fiberoptic biosensors.
• Halophenols can enhance peroxidase-catalyzed luminol chemiluminescence, enabling specific analyte detection.
• Electrochemical triggering of light emission is feasible for biosensors utilizing oxidase enzymes.

Purpose of the Study:

• To develop fiberoptic biosensors for detecting chlorophenols using chemiluminescence.
• To create electrochemiluminescent fiberoptic biosensors for glucose and lactate detection.
• To evaluate the sensitivity and dynamic range of the developed biosensor systems.

Main Methods:

• Immobilization of horseradish peroxidase on a collagen membrane for chlorophenol sensing.
• Testing ten different chlorophenols with the chemiluminescent sensor.
• Immobilization of glucose oxidase and lactate oxidase on polyamide membranes for electrochemiluminescent sensing.
• Electrochemical triggering of light emission using a glassy carbon electrode.

Main Results:

• A chlorophenol sensor achieved a lower detection limit of 0.01 microM for 4-chloro-3-methylphenol.
• Electrochemical fiberoptic biosensors for glucose and lactate were successfully developed.
• Detection limits for glucose and lactate were 150 pmol and 60 pmol, respectively.
• Linear dynamic ranges were established for both glucose and lactate detection.

Conclusions:

• Fiberoptic biosensors based on luminol chemiluminescence and electrochemiluminescence are effective for analyte detection.
• The developed sensors demonstrate high sensitivity and suitable dynamic ranges for practical applications.
• Immobilized enzymes on membranes provide a robust platform for biosensor development.