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Resolution of positional isomers by capillary electrochromatography
1Development Chemistry 5, Chemical Development Division, GlaxoWellcome Medicines Research Centre, Stevenage, Herts, UK.
Journal of Chromatography. A
|February 24, 2001
Summary
Capillary electrochromatography (CEC) effectively separates critical positional isomers, crucial for pharmaceutical impurity analysis. This method offers a viable alternative to gas chromatography and nano-high-performance liquid chromatography.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Electroseparation techniques enhance analytical efficiency and reduce time.
- Capillary electrophoresis (CE) has spurred advancements in electrochromatography.
Purpose of the Study:
- To evaluate capillary electrochromatography (CEC) for separating positional isomers.
- To compare CEC with gas chromatography (GC) and nano-high-performance liquid chromatography (nano-HPLC).
Main Methods:
- Capillary electrochromatography (CEC)
- Gas chromatography (GC)
- Nano-high-performance liquid chromatography (nano-HPLC)
Main Results:
- CEC demonstrated effective separation of two positional isomers.
- Comparison highlights CEC's performance relative to GC and nano-HPLC.
Conclusions:
- Capillary electrochromatography is a valuable tool for resolving critical positional isomers.
- Accurate isomer separation is vital for identifying impurities in pharmaceutically active drugs.