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Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis
1Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA. fstahl@morel.uoregon.edu
Molecular & General Genetics : MGG
|February 24, 2001
Abstract:
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.