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Expression of matrix metalloproteinases in patients with acute myocardial infarction
Abstract:
This study investigated the clinical significance of matrix metalloproteinases (MMPs) in acute myocardial infarction (AMI) and the involvement of peripheral blood mononuclear cells (PBMCs), which are a possible source of MMPs in AMI. Forty patients with AMI were recruited. Plasma and PBMCs were isolated from peripheral blood on days 1, 7, 14 and 21 after the onset of AMI. Levels of MMP-1 and MMP-2 were measured by enzyme-linked immunosorbent assay. The MMP-1 level in the culture medium of PBMCs after incubation for 24h was designated as 'PBMC-MMP-1 level.' Plasma MMP-1 did not significantly change during the course of AMI, but the plasma MMP-2 levels increased gradually after the onset of AMI with maximum elevation on day 21 after onset. Plasma MMP-2 activity also became significantly elevated during the course of AMI. PBMC-MMP-1 levels in the patients were significantly higher than those in control subjects over the course of AMI. Significant positive correlations were observed between maximum PBMC-MMP-1 levels and maximum plasma C-reactive protein levels (r=+0.55, p<0.01) and left ventricular end-diastolic volume index (r=+0.63, p<0.001). In conclusion, plasma MMP-2 levels and activity and MMP-1 production by PBMCs are increased in patients with AMI. Inflammation after AMI may enhance production of MMP-1 by PBMCs. These changes may play an important role in the ventricular remodeling that occurs after AMI by promoting the degradation of the extracellular matrix.
Insights
Matrix metalloproteinases (MMPs) are elevated in acute myocardial infarction (AMI) patients, with increased MMP-2 in plasma and MMP-1 produced by peripheral blood mononuclear cells (PBMCs). These MMPs may contribute to ventricular remodeling post-AMI.
Area of Science:
- Cardiology
- Biochemistry
- Immunology
Background:
- Matrix metalloproteinases (MMPs) play a role in extracellular matrix degradation.
- Peripheral blood mononuclear cells (PBMCs) are a potential source of MMPs.
- Understanding MMP involvement in acute myocardial infarction (AMI) is crucial for clinical insights.
Purpose of the Study:
- To investigate the clinical significance of MMP-1 and MMP-2 in AMI.
- To determine the role of PBMCs as a source of MMPs in AMI.
- To explore the relationship between MMPs, inflammation, and ventricular remodeling after AMI.
Main Methods:
- Plasma and PBMCs were collected from 40 AMI patients on days 1, 7, 14, and 21 post-onset.
- MMP-1 and MMP-2 levels were quantified using enzyme-linked immunosorbent assay.
- PBMC-derived MMP-1 (PBMC-MMP-1) was measured after 24h incubation.
Main Results:
- Plasma MMP-2 levels and activity progressively increased after AMI onset, peaking on day 21.
- PBMC-MMP-1 levels were significantly higher in AMI patients compared to controls.
- Elevated PBMC-MMP-1 correlated positively with C-reactive protein and left ventricular end-diastolic volume index.
Conclusions:
- AMI is associated with increased plasma MMP-2 and enhanced MMP-1 production by PBMCs.
- Inflammation following AMI may stimulate PBMC-derived MMP-1 production.
- These MMP alterations are implicated in the extracellular matrix degradation driving post-AMI ventricular remodeling.