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Related Experiment Videos

Advances in process chromatography gradient elution using binary linear gradients.

V Natarajan1, G Purdom

  • 1Millipore Corporation, BioProcess Division, Bedford, MA 01730, USA.

Journal of Chromatography. A
|February 24, 2001
PubMed
Summary
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Linear gradients in chromatography offer theoretical advantages but face implementation challenges. This study presents a novel feedforward control strategy for accurate, reliable, and reproducible process-scale linear gradients.

Area of Science:

  • Chemical Engineering
  • Analytical Chemistry
  • Biotechnology

Background:

  • Chromatography techniques commonly use mobile phase gradients for separation.
  • Process-scale chromatography predominantly employs stepwise or isocratic gradients.
  • Linear gradients offer theoretical performance benefits but have been limited by equipment concerns.

Purpose of the Study:

  • To address the challenges in implementing linear gradients at process scale.
  • To present a novel feedforward control strategy for accurate linear gradient generation.
  • To demonstrate the practical application and performance of this control strategy.

Main Methods:

  • Development of a simple feedforward control strategy.
  • Implementation of the control strategy on a commercial process-scale system.

Related Experiment Videos

  • Validation of gradient accuracy, reliability, and reproducibility.

    • Main Results:

    • The feedforward control strategy enables accurate process-scale linear gradients.
    • Achieved better than +/- 2% accuracy for process-scale linear gradients.
    • Demonstrated practical heuristics and implementation details of the control strategy.

    Conclusions:

    • The developed feedforward control strategy overcomes previous limitations in linear gradient generation.
    • This advancement facilitates the adoption of linear gradients for improved process performance and economics.
    • The system provides accurate and reproducible linear gradients essential for process-scale chromatography.