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[Foundational study of subcritical water chromatography]
1Department of Pharmaceutical Analysis, Second Military Medical University, Shanghai 200433, China.
Yao Xue Xue Bao = Acta Pharmaceutica Sinica
|February 24, 2001
Summary
Subcritical water chromatography effectively separates medium to high polarity compounds. Increasing temperature enhances separation efficiency and peak shape, especially with thermostable capillary columns.
Area of Science:
- Analytical Chemistry
- Chromatography
Context:
- Subcritical water chromatography (SWC) is an emerging separation technique.
- Investigating SWC for medium and high polarity compounds is crucial for expanding its applications.
Purpose:
- To evaluate the chromatographic behavior of medium and high polarity compounds using SWC.
- To explore the use of packed and capillary columns in SWC.
- To study the effects of temperature and pressure on separation.
Summary:
- A SWC system with FID detection was developed for packed and capillary columns.
- Six alcohols were separated on a C18 column, and temperature effects were analyzed.
- Benzoic acid and gensenoside Rg1 were studied on a SE-54 capillary column under varying temperature and pressure.
Impact:
- Temperature programming in SWC enables selective separation of diverse polarity compounds.
- Thermostable capillary columns offer a wider operational temperature range, enhancing SWC capabilities.