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Published on: May 31, 2008
Investigation of avenacin-deficient mutants of Avena strigosa
M R Trojanowska1, A E Osbourn, M J Daniels
1Department of Biological Sciences, University of Hull, UK. miranda.trojanowska@bbsrc.ac.uk
Abstract:
The biosynthesis of cyclic triterpenoids in ten saponin-deficient (sad) mutant varieties of the diploid oat Avena strigosa is reported. Two mutants were found to be deficient in 2,3-oxidosqualene:beta-amyrin cyclase (OSbetaAC) (EC 5.4.99) and thus unable to produce the beta-amyrin necessary for the production of avenacins. The other mutants studied had post beta-amyrin lesions. 2,3-Oxidosqualene:cycloartenol cyclase (OSCC) (EC 5.4.99.8) needed for sterol formation was present in all ten mutants.
