Related Experiment Videos
Analysis of phytochelatins in plant matrices by pre-column derivatization, high-performance liquid chromatography and
S Döring1, S Korhammer, M Oetken
1Internationales Hochschulinstitut Zittau, Lehrstuhl Umweltverfahrenstechnik, Zittau, Germany.
Fresenius' Journal of Analytical Chemistry
|February 28, 2001
Abstract:
A sensitive method for the determination of phytochelatins in plant matrices by pre-column derivatization with monobromobimane (mBrB) and high-performance liquid chromatography (HPLC) on reversed phases and fluorescence-detection has been developed and applied to cucumber sprouts (Cucumis sativus) treated with cadmium and to the water moss Fontinalis antipyretica (Cd in environmentally-relevant concentrations). Whereas phytochelatins were found in the Cd-treated sprouts, no phytochelatins were detected in Fontinalis anitipyretica.