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Related Experiment Videos

Immunochromatographic techniques--a critical review.

M G Weller1

  • 1Institute of Hydrochemistry, Technical University of Munich, München, Germany. michael.weller@ch.tum.de

Fresenius' Journal of Analytical Chemistry
|February 28, 2001
PubMed
Summary

This review explores combining biochemical immunoassays with instrumental chromatography. It highlights various coupled systems and components for complex analytical challenges.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Hyphenated techniques combining biochemical and instrumental methods are gaining popularity.
  • However, the integration of immunoassays with chromatography faces challenges in widespread acceptance.

Purpose of the Study:

  • To critically review implemented and potential options for coupled immunoassay-chromatography systems.
  • To discuss the performance of immunochromatographic systems in solving complex analytical problems.

Main Methods:

  • Review of various coupled systems and components: immunoaffinity extraction, immunoaffinity chromatography, immunochemical detectors, immunoblotting, receptor assays, enzyme inhibition assays, displacement assays, flow-injection immunoassays.
  • Discussion of stationary phases like restricted access materials and molecularly imprinted polymers.
  • Evaluation of immunochromatographic systems' capabilities.

Main Results:

  • Identified various techniques and components for integrating immunoassays with chromatography.
  • Highlighted the potential of these hyphenated systems for complex analytical tasks.
  • Discussed the performance characteristics of immunochromatographic systems.

Conclusions:

  • Despite challenges, coupled immunoassay-chromatography systems offer significant potential for advanced analytical applications.
  • Further development and acceptance of these hyphenated techniques are crucial for addressing demanding analytical problems.

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