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Imaging the activity of nitrate reductase by means of a scanning electrochemical microscope
J Zaumseil1, G Wittstock, S Bahrs
1Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, University of Leipzig, Germany.
Abstract:
Scanning electrochemical microscopy (SECM) was used to characterize immobilized nitrate reductase (NaR) from Pseudonomonas stutzeri (E.C. 1.7.99.4). Nitrate reductase with membrane fragment was embedded in a polyurethane hydrogel in a capillary and solubilized NaR without membrane fragment was covalently coupled to a diaminoethyl-cellulose-carbamitate film on glass. After systematic studies of possible mediators, SECM feedback imaging of both forms of immobilized NaR was accomplished with methylviologen as redox mediator.
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