Semi-automated colorimetric determination of triglycerides

1. A semi-automated procedure for the determination of triglycerides based on the manual procedure of Fletcher and the semi-automated procedure of Kessler and Lederer has been developed. 2. Reaction conditions and flow-rates have been optimized to result in a trouble-free procedure which produces 99+ per cent recovery on added recovery tests, and a standard deviation of 4.8 on specimens in the low normal range. 3. An inexpensive scale expansion device is used to increase accuracy by improving chart readability. 4. Analysis of commercial control sera produced results which correlated well with stated values.