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Mutations in the alpha-synuclein gene in Parkinson's disease among Indians
S Nagar1, R C Juyal, S Chaudhary
1Department of Genetics, University of Delhi, New Delhi, India.
Objective:
To investigate the prevalence of G88C, G209A and any other mutation(s) in exons 3 and 4 of the alpha-synuclein gene in Indian patients with Parkinson's disease (PD).
Methods:
A total of 169 PD patients comprising 18 familial, 3 juvenile, 48 early onset and 100 sporadic cases were included in this study. Genomic DNA was amplified by PCR using primers specific for Exons 3 and 4. Mutations at G88C and G209A were screened following restriction enzyme digestion of the PCR product. Direct PCR product sequencing of entire exons 3 and 4 was carried out for at least one proband each from the 10 familial cases.
Results:
Neither G88C and G209A mutations nor any other mutation in exons 3 and 4 was found in the PD patients analysed.
Conclusion:
The G88C and G209A mutations do not seem to be the predominant genetic determinant of PD among Indians.