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Techniques to Induce and Quantify Cellular Senescence
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Strategies for evaluation of signaling pathways and transcription factors altered in aging

N K Fukagawa1, C R Timblin, S Buder-Hoffman

  • 1Department of Medicine, University of Vermont College of Medicine, Burlington 05405-0068, USA. nfukagaw@zoo.uvm.edu

Antioxidants & Redox Signaling
|March 7, 2001
PubMed

Insights

Oxidative stress contributes to aging and age-related diseases like diabetes and atherosclerosis. This review explores how to assess redox-sensitive pathways and transcription factors involved in cellular dysfunction.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Aging involves accumulating oxidative damage to cellular components.
  • Oxidative stress triggers cell signaling pathways affecting cell function and fate.
  • Aging, diabetes mellitus, and atherosclerosis share common oxidant-related signaling pathways.

Purpose of the Study:

  • To review methods for evaluating key redox-sensitive signaling pathways.
  • To examine transcription factors altered by aging, diabetes, and atherosclerosis.
  • To understand the role of oxidative stress in age-related cellular dysfunction.

Main Methods:

  • Literature review of studies on oxidative stress and signaling pathways.
  • Analysis of research on transcription factor activation in aging and disease.
  • Focus on redox-sensitive pathways and their modulation.

Main Results:

  • Oxidative stress impacts DNA, RNA, proteins, lipids, and carbohydrates.
  • Signaling cascades initiated by oxidative stress modulate cell growth and death.
  • Abnormal transcription factor activation is linked to cellular dysfunction in aging and disease.

Conclusions:

  • Oxidative stress is a central mechanism in aging and related diseases.
  • Understanding redox-sensitive pathways is crucial for addressing age-related cellular dysfunction.
  • Targeting these pathways may offer therapeutic strategies for aging and associated conditions.

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