Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bile acids induce eosinophil degranulation by two different mechanisms.

K Yamazaki1, G J Gleich, H Kita

  • 1Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA.

Hepatology (Baltimore, Md.)
|March 7, 2001
PubMed
Summary

Bile acids directly activate eosinophils, a key immune cell in bile duct diseases. This study reveals how specific bile acids trigger eosinophil degranulation and cytokine release, offering new insights into immune cholangiopathies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Memory and Celebration: Dr. James J. Lee.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
Same author

Repeat treatment of acute hereditary angioedema attacks with open-label icatibant in the FAST-1 trial.

Clinical and experimental immunology·2014
Same author

Eosinophil major basic protein activates human cord blood mast cells primed with fibroblast membranes by integrin-β1.

Allergy·2013
Same author

The consequences of not having eosinophils.

Allergy·2013
Same author

Eosinophil peroxidase induces the expression and function of acid-sensing ion channel-3 in allergic rhinitis: in vitro evidence in cultured epithelial cells.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2012
Same author

The role of eosinophil major basic protein in angiogenesis.

Allergy·2009

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Eosinophils and bile acids are implicated in immune cholangiopathies.
  • Bile acids may interact with immune cells near damaged bile ducts.
  • The direct effect of bile acids on eosinophils remains unexplored.

Purpose of the Study:

  • To investigate if bile acids directly activate eosinophils.
  • To determine the mechanisms of bile acid-induced eosinophil effector functions.

Main Methods:

  • In vitro assays using human eosinophils.
  • Exposure to varying concentrations of taurine-conjugated chenodeoxycholic acid (TCDCA) and taurine-conjugated ursodeoxycholic acid.
  • Assessment of eosinophil degranulation, superoxide production, IL-8 release, cell morphology, and viability.

Related Experiment Videos

  • Use of tyrosine kinase and microfilament inhibitors.

    • Main Results:

    • Hydrophobic TCDCA induced eosinophil degranulation via two distinct mechanisms: regulated release at lower concentrations and passive cytolysis at higher concentrations.
    • Low to moderate TCDCA concentrations stimulated superoxide and IL-8 production, indicative of active eosinophil function.
    • Hydrophilic taurine-conjugated ursodeoxycholic acid also activated eosinophils, but with lower potency than TCDCA.

    Conclusions:

    • Bile acids are direct activators of human eosinophils.
    • Concentration-dependent mechanisms explain bile acid-induced eosinophil degranulation and effector functions.
    • These findings provide novel insights into the role of bile acids in immune cholangiopathies.