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Related Concept Videos

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Published on: October 27, 2011

DNA replication control through interaction of E2F-RB and the origin recognition complex.

G Bosco1, W Du, T L Orr-Weaver

  • 1Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.

Nature Cell Biology
|March 7, 2001
PubMed
Summary

The E2F transcription factor and retinoblastoma protein (Rbf) limit DNA replication. These proteins interact with the origin recognition complex (DmORC) at replication origins to control cell cycle progression.

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Last Updated: Jun 26, 2026

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The E2F transcription factor and retinoblastoma protein are key regulators of cell-cycle progression and DNA replication.
  • Disruptions in these pathways are implicated in uncontrolled cell proliferation.

Purpose of the Study:

  • To investigate the roles of Drosophila dE2F1, dDP, and Rbf in DNA replication.
  • To elucidate the interaction between these proteins and the origin recognition complex (DmORC) at replication origins.

Main Methods:

  • Analysis of Drosophila mutants for Rbf, dE2F1, and dDP.
  • Investigating protein complex formation using co-immunoprecipitation.
  • In vivo binding assays to determine protein-DNA interactions at the chorion gene origin.

Main Results:

  • Rbf mutants exhibit a failure to limit DNA replication.
  • dDP, dE2F1, and Rbf proteins form a complex with DmORC.
  • dE2F1 and DmORC bind to the chorion origin of replication in vivo.

Conclusions:

  • dE2F1 and Rbf function collaboratively at replication origins.
  • These proteins interact with DmORC to limit DNA replication.
  • This interaction is crucial for regulating DNA replication initiation.