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[Endonuclease from Proteus mirabilis]
Z Z Salikhova1, R B Sokolova, A Z Ponomareva
1Kazan State University, Kazan, 420008 Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|March 10, 2001
Summary
Proteus mirabilis secretes a potent endonuclease, Pm endonuclease, with both DNase and RNase activity. This enzyme efficiently cleaves DNA and requires specific conditions for optimal function.
Area of Science:
- Microbiology
- Enzymology
Background:
- Proteus mirabilis possesses nucleases with DNase and RNase activities.
- These nucleases are found in the culture liquid and periplasm.
Purpose of the Study:
- To isolate and purify the nuclease from Proteus mirabilis.
- To characterize the enzymatic properties of the purified nuclease, designated Pm endonuclease.
Main Methods:
- Isolation of the enzyme from the periplasm of Proteus mirabilis.
- Purification to a functionally homogeneous state.
- Enzymatic assays to determine substrate specificity and optimal conditions.
Main Results:
- The purified enzyme, Pm endonuclease, exhibits potent DNase and RNase activities.
- It cleaves both denatured and native DNAs via an endonuclease mechanism.
- Optimal activity occurs at pH 10.4-10.6, is temperature-dependent, requires bivalent metal ions, and is inhibited by citrate and EDTA.
Conclusions:
- Proteus mirabilis produces a versatile endonuclease with significant DNase and RNase capabilities.
- Pm endonuclease is a novel enzyme with specific biochemical requirements, offering potential for biotechnological applications.