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[New full length cDNA cloned from normal pituitary and pituitary tumors]
1Shanghai Institute of Endocrinology & Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025 China.
Objective:
To clone new full length cDNA from normal pituitary and pituitary tumors.
Methods:
cDNA libraries of human normal pituitary and pituitary tumors were constructed, and large-scale sequencing and bioinformatics were used. Silico cloning and rapid amplification of cDNA ends (RACE) were also used.
Results:
67 new full length cDNAs were cloned: 41 were cloned from normal pituitary, and 26 from pituitary tumors. Of the 67 cDNAs, 16 were related with endocrine function; at least 2 with a classical signal peptide were secretory protein, and 3 with classical transmembrane domains were transmembrane protein. Among the 5 cloned new full length cDNAs involved in signal transduction pathway of hormone, at least 2 participated in signal transduction pathway of receptors with tyrosine kinase activition.
Conclusion:
During short time, 67 new full length cDNA were cloned from normal pituitary and pituitary tumors by large-scale producing expressed sequence tags. Some of them may play an important role in the regulation of physiological and pathological processes of pituitary.